Amylosucrase from Neisseria polysaccharea: novel catalytic properties

FEBS Letters

Willemot, René-Marc¹  Remaud-Simeon, Magali¹  Monsan, Pierre¹  Planchot, Véronique²  Potocki de Montalk, Gabrielle¹  Sarçabal, Patricia¹ 

[1] Centre de Bioingénierie Gilbert Durand, UMR CNRS 5504, UMR INRA 792, INSA, Complexe Scientifique de Rangueil, 31077 Toulouse Cedex 4, France;INRA, URPOI, 44316 Nantes Cedex 3, France

关键词: Amylosucrase;    Sucrose;    α-1;    4-Glucan;    Oligosaccharide;    Non-Michaelian profile;   

DOI  :  10.1016/S0014-5793(00)01406-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Amylosucrase is a glucosyltransferase that synthesises an insoluble α-glucan from sucrose. The catalytic properties of the highly purified amylosucrase from Neisseria polysaccharea were characterised. Contrary to previously published results, it was demonstrated that in the presence of sucrose alone, several reactions are catalysed, in addition to polymer synthesis: sucrose hydrolysis, maltose and maltotriose synthesis by successive transfers of the glucosyl moiety of sucrose onto the released glucose, and finally turanose and trehalulose synthesis – these two sucrose isomers being obtained by glucosyl transfer onto fructose. The effect of initial sucrose concentration on initial activity demonstrated a non-Michaelian profile never previously described.

【 授权许可】

Unknown