Rapid validation of molecular structures of biological samples by electrospray‐mass spectrometry

FEBS Letters

Oliver, R.W.A.²  Ashton, D.S.¹  Beddell, C.R.¹  Green, B.N.³ 

[1] The Wellcome Research Laboratories, Langley Court, Beckenham, Kent BR3 3BS, UK;BMA Research Unit, University of Salford, Salford, M5 4WT, UK;VG Biotech, Tudor Road, Altrincham, Cheshire, WA14 5RZ, UK

关键词: Molecular mass;    Electrospray ionisation mass spectrometry;    Effective accuracy;    Protein;    Glycoprotein;   

DOI  :  10.1016/0014-5793(94)80572-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A short account is presented of the method of measuring molecular masses (M _r) of pure biological samples by electrospray ionisation mass spectrometry. It is demonstrated that the technique yields M _r values with an effective accuracy equal to or better than 0.008% of the calculated M _r, provided that the correct molecular structure is employed in the calculation. It is therefore recommended that this method of measuring M _r's should be considered to form an essential part of all studies aimed at elucidating the molecular structure of purified biological macromolecules or for confirming the identity of labelled samples of such molecules.

【 授权许可】

Unknown