A comparison of the molecular specificities of whole cell and endonuclear phosphatidylcholine synthesis

FEBS Letters

Clark, Graeme T¹  Neale, James R¹  Postle, Anthony D¹  Hunt, Alan N¹ 

[1] Mailpoint 803, Allergy and Inflammation Sciences, Division of Infection, Inflammation and Repair, School of Medicine, University of Southampton, Level G, Centre Block, Southampton General Hospital, Tremona Road, Southampton SO16 6YD, UK

关键词: Phosphatidylcholine synthesis;    IMR-32 nuclei;    Deuterium labelling;    Electrospray ionisation mass spectrometry;    AA;    arachidonic acid (20:4;    n-6);    choline-d9;    9 deuterated methyl choline;    DAG;    diacylglycerol;    DHA;    docosahexaenoic acid (22:6;    n-3);    ESI-MS;    electrospray ionisation mass spectrometry;    PtdCho;    phosphatidylcholine;    PtdIns;    phosphatidylinositol;    PUFA;    polyunsaturated fatty acid;   

DOI  :  10.1016/S0014-5793(02)03429-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Deuterated choline-d₉ labelling of IMR-32 cells enabled comparison of the molecular specificities of whole cell and endonuclear phosphatidylcholine synthesis after 96 h polyunsaturated fatty acid supplementation. Surprisingly, while cell phosphatidylcholine synthesis and remodelling reflected a pattern of polyunsaturated fatty acid accretion, the saturated endonuclear phosphatidylcholine pool was only transiently labelled with polyunsaturates. Periodic endonuclear accumulations of the lipid second messenger diacylglycerol, mobilised from unsaturated phosphatidylinositol or saturated phosphatidylcholine, accompany cell proliferation. Non-specific incorporation into endonuclear phosphatidylcholine and selective removal or remodelling of unsaturated molecular species may form part of a single ‘off switch’ recycling all endonuclear diacylglycerol accumulations.

【 授权许可】

Unknown