A porcine gut polypeptide identical to the pancreatic hormone PP (pancreatic polypeptide)

FEBS Letters

Höög, Anders³  Chen, Zheng-wang²  Östenson, Claes-Göran¹  Mutt, Viktor²  Carlquist, Mats⁴  Bergman, Tomas²  Efendic, Suad¹  Näslund, Jan⁵  Norberg, Åke²  Jörnvall, Hans² 

[1] Department of Endocrinology and Clinical Genetics, Karolinska Hospital, S-171 76 Stockholm, Sweden;Department of Medical Biochemistry and Biophysics, Karolinska Institutet, S-171 77 Stockholm, Sweden;Department of Pathology, Karolinska Hospital, S-171 76 Stockholm, Sweden;KaroBio AB, S-141 04 Huddinge, Sweden;Department of Alcohol and Drug Addiction Research, Karolinska Hospital, S-171 76 Stockholm, Sweden

关键词: Pancreatic hormone;    Pancreatic polypeptide;    Pig intestine;    Radioimmunoassay;    Immunohistochemistry;   

DOI  :  10.1016/0014-5793(94)80464-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A peptide hormone has been isolated from porcine intestine. Its primary structure was found to consist of 36 amino acid residues in a sequence identical to that of the porcine pancreatic polypeptide, previously not isolated from intestines or a tissue other than pancreas. The gut polypeptide significantly suppresses glucose-induced insulin secretion in vitro. Using an immunohistochemical technique, we also identified cells in the porcine gastrointestinal tract that were immunoreactive with pancreatic polypeptide. The immunoreactivity disappeared after absorption with the isolated gut polypeptide or synthetic human pancreatic polypeptide.

【 授权许可】

Unknown