An unusual metal‐binding cluster found exclusively in the avian breast muscle troponin T of Galliformes and Craciformes

FEBS Letters

Jin, Jian-Ping¹  Smillie, Lawrence B.¹ 

[1] MRC Group in Protein Structure and Function, Department of Biochemistry, University of Alberta, Edmonton, Alberta, T6G 2H7, Canada

关键词: Metal-binding cluster;    Troponin T;    Zinc;    Galliformes;    Breast muscle;    Affinity purification;   

DOI  :  10.1016/0014-5793(94)80256-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A repeating metal-binding (Cu²⁺ > Ni²⁺ > Zn²⁺ ~ Co²⁺) sequence (HE/AEAH)₄ has been identified in troponin T isoforms specifically expressed in the breast but not leg muscles of all Galliformes and Craciformes. It is absent in the skeletal and cardiac muscles of mammals and all other avian species investigated. Concentration of the metal-binding sites is adequate to affect free metal levels in the muscle cell and we suggest a possible link between its presence in breast muscle of Galliformes and the high ratio of breast muscle to total body muscle mass and explosive but short-lived flight pattern of these birds. This sequence can be used for a highly selective metal-affinity chromatographic purification of muscle or engineered TnTs even in high salt and/or urea.

【 授权许可】

Unknown