FEBS Letters | |
Photophobic responses and phototaxis in Chlamydomonas are triggered by a single rhodopsin photoreceptor | |
Hegemann, Peter1  Kröger, Petra1  | |
[1] Institut für Biochemie, Universität Regensburg, 93040 Regenburg, Germany | |
关键词: Rhodopsin; Phototaxis; Blue light receptor; Chlamydomonas reinhardtii; | |
DOI : 10.1016/0014-5793(94)80229-7 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
The rhodopsin nature of the photoreceptor for the behavioural light responses in Chlamydomonas has originally been revealed by action spectroscopy. Meanwhile most physiological experiments and the identification of all-trans-retinal in cell extracts favour that this chlamyrhodopsin contains an all-trans-type retinal chromophore with strong similarity to the light sensors SR I and SRII from Halobacteria. Reconstitution of retinal-deficient cells with [3H]retinal identified a single retinal protein with a MW of 30,000. Chlamyrhodopsin triggers a photoreceptor current in the eyespot region resulting in direction changes or phototaxis. Furthermore, when the light stimulus oversteps a critical level, two flagellar currents appear, which are the basis for photophobic responses. The physiological, electrophysiological and biochemical experiments suggest that all behavioural responses are triggered by a single rhodopsin-type receptor.
【 授权许可】
Unknown
【 预 览 】
Files | Size | Format | View |
---|---|---|---|
RO201912020299280ZK.pdf | 503KB | download |