The detection and characterization of G‐proteins in the eyespot of Chlamydomonas reinhardtii

FEBS Letters

Garnovskaya, M.N.²  Korolkov, S.N.²  Chunaev, A.S.¹  Basov, A.S.¹  Dumler, I.L.² 

[1] Leningrad State University, Universitetskaya nab. 7/9, 199034 Leningrad, USSR;Sechenov Institute of Evolutionary Physiology and Biochemistry, USSR Academy of Sciences, M. Torezpr. 44, 194223 Leningrad, USSR

关键词: G-protein;    GTPase;    Rhodopsin;    GTPγ[35S];    Chlamydomonas reinhardtii;   

DOI  :  10.1016/0014-5793(90)81251-I

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The presence of G-proteins in the eyespot fraction of Chlamydomonas reinhardtii is shown. This fraction is capable of binding (GTPγ[³⁵S], possesses the GTPase activity and interacts with antibodies raised against a highly conserved peptide of most G-proteins' α-subunit. Cross-reaction with a 24-kDa protein is detected on immunoblots. Using an antiserum prepared from vertebrate β-subunit peptide, two additional proteins with apparent M _r 21 and 29 kDa could be revealed. The light-dependence of GTPase extraction from eyespot membranes is shown. The results make it possible to suggest the participation of G-proteins in the photosensory transduction chain of Ch. reinhardtii.

【 授权许可】

Unknown