A sialic acid‐binding lectin from the mushroom Hericium erinaceum

FEBS Letters

Chiba, Seiya¹  Kawagishi, Hirokazu²  Kimura, Atsuo¹  Uno, Akinori²  Mori, Hironobu² 

[1]Department of Applied Bioscience, Faculty of Agriculture, Hokkaido University, Sapporo 060, Japan

[2]Department of Applied Biological Chemistry, Faculty of Agriculture, Shizuoka University, 836 Ohya, Shizuoka 422, Japan

关键词: Lectin;    sialic acid-binding;    Purification;    Mushroom;    Fungus;    Hericium erinaceum;    BSM;    bovine submaxillary mucin;    HEL;    Hericium erinaceum lectin;    NeuAc;    N-acetylneuraminic acid;    NeuGc;    N-glycolylneuraminic acid;    PBS;    10 mM phosphated-buffered saline (pH 7.4);    SDS-PAGE;    sodium dodecylsulfate-polyacrylamide gel electrophoresis;    all sugars are of d-configuration;    unless otherwise stated;   

DOI  :  10.1016/0014-5793(94)80172-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A lectin was isolated from the mushroom Hericium erinaceum. This lectin is composed of two different subunits of 15 and 16 kDa and the molecular mass of the intact lectin was estimated to be 54 kDa by gel filtration. It exhibits specificity towards sialic acids, especially N-glycolylneuraminic acid.

【 授权许可】

Unknown