Affinity chromatography of Vicia graminea (blood group N) lectin using concanavalin A—Sepharose

FEBS Letters

Prigent, M.J.¹  Lombart, C.²  Asseraf, A.³ 

[1]Biologie et Pathologie Moléculaires des Glycoprotéines, Unité INSERM 180, Faculté de Médecine, 45, rue des Saints-Pères, 75006 Paris, France

[2]Laboratoire de Biochimie, UER Biomédicale des Saints-Pères, 45, rue des Saints-Pères, 75006 Paris France

[3]Laboratoire de Biochimie Génétique, INSERM U76, Centre National de Transfusion Sanguine, 6, rue Alexandre Cabanel, 75739 Paris Cedex 15, France

关键词: Vivia graminea seeds;    Lectin;    Blood Group N;    Concanavalin A—Sepharose;    Affinity chromatography;    Purification;   

DOI  :  10.1016/0014-5793(82)81309-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The lectin extracted from Vicia graminea seeds has been purified by conventional techniques but such procedures did not give a satisfactory yield. We describe a new purification which involves 3 steps after obtention of the crude extract. The first step is based on affinity chromatography on con A—Sepharose. Further purification steps were performed on DEAE-Sephacel chromatography and ultrogel AcA₄₄ gel filtration. The homogeneity of the lectin was demonstrated by polyacrylamide gel electrophoresis. Purification of the lectin by this new method was less time consuming, the yield was higher and the specific activity increased.

【 授权许可】

Unknown