Effects of calcium ions on proteolytic processing of HIV‐1 gp160 precursor and on cell fusion

FEBS Letters

Bahraoui, Elmostafa¹  Montagnier, Luc²  Moulard, Maxime¹ 

[1] Laboratoire de Biochimie, CNRS URA 1455 - Ingénierie des Protéines, Faculté de Médecine Secteur Nord, Bd Pierre Dramard, 13916 Marseille Cedex 20, France;CNRS URA 1157, Institut Pasteur, 28 rue du Dr Roux, 75724 Paris Cedex 15, France

关键词: HIV-1;    Processing;    Glycoprotein;    Endoprotease;    Calcium ion;   

DOI  :  10.1016/0014-5793(94)80284-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Complete activation of human immunodeficiency virus type 1 (HIV-1) requires the endoproteolytic cleavage by cellular protease of the envelope glycoprotein precursor (gp160) into the external glycoprotein gp120, and the transmembrane glycoprotein gp41. We report here the effect of depletion of cellular calcium ions on maturation of precursor gp160 and its concomitant effect on syncytium formation. We show that the cellular endoprotease activity responsible for gp160 maturation and the capacity for HIV-1 to induce syncytium formation are calcium-dependent. In addition, we show that endoproteolytic maturation is a key step in syncytium formation induced by HIV-1.

【 授权许可】

Unknown