Effect of neutral and acidic phospholipids on mitochondrial ATP synthase secondary structure

FEBS Letters

Lippe, Giovanna³  Loregian, Arianna¹  Bertoli, Enrico²  Tanfani, Fabio²  Sala, Federica Dabbeni¹ 

[1] Dipartimento di Farmacologia, Università di Padova, Largo Meneghetti, Padova, Italy;Istituto di Biochimica, Facoltà di Medicina e Chirurgia, Università di Ancona, Via Ranieri, 60131 Ancona, Italy;Dipartimento di Scienze e Tecnologie, Università di Udine, Via Gervasutta 48, Udine, Italy

关键词: ATP synthase;    Infrared spectroscopy;    Protein secondary structure;    Egg phosphatidylcholine;    Asolectin;    Liposome;    egg-PC;    egg phosphatidylcholine;    ATPase;    delipidated ATP synthase;    aso/ATPase;    asolectin-reconstituted ATP synthase;    egg-PC/ATPase;    egg-PC-reconstituted ATP synthase;    ANC;    adenine nucleotide carrier;   

DOI  :  10.1016/0014-5793(93)80859-S

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The secondary structure of delipidated and egg phosphatidylcholine or asolectin reconstituted mitochondrial ATP synthase complex from beef heart was investigated by Fourier transform infrared spectroscopy. Upon reconstitution, the infrared spectra of ATP synthase revealed an increase in turns and a concomitant decrease in β-sheet content which occurred to a larger extent in the presence of asolectin rather than in the presence of egg phosphatidylcholine. These data correlate with kinetic data showing a higher ATPase activity of the asolectin reconstituted enzyme protein than the egg phosphatidylcholine reconstituted or delipidated enzyme complexes.

【 授权许可】

Unknown