cDNA cloning of rat proteasome subunit RC10‐II, assumed to be responsible for trypsin‐like catalytic activity

FEBS Letters

Tanaka, Keiji²  Akioka, Hiroshi²  Tokunaga, Fuminori³  Tamura, Tomohiro²  Ichihara, Akira²  Nishimura, Chihiro¹ 

[1] Laboratory of Applied Toxicology, Nippon Kayaku Co. Ltd., 239 Iwahana-machi, Takasaki-city, Gunma, 370-12, Japan;Institute for Enzyme Research, The University of Tokushima, Tokushima 770, Japan;Himeji Institute of Technology, Faculty of Science, Harima Science Park City, Hyogo 678-12, Japan

关键词: cDNA cloning;    Multicatalytic proteinase;    Proteasome;    Subunit RC 10-II;    Trypsin-like activity;   

DOI  :  10.1016/0014-5793(93)80856-P

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The nucleotide sequence of a cDNA that encodes anew subunit, named RC10-II, of the 20S proteasome of rat embryonic brain has been determined. The polypeptide predicted from the open reading frame consists of 205 amino acid residues with a calculated molecular weight of 22,965 and isoelectric point of 6.15. Computer analysis showed that RC10-II belongs to the β-type subgroup of proteasomes, differing clearly from α-type subunits of the proteasome gene family. The primary structure of RC10-II was found to contain the partial amino acid sequences of several fragments of subunit 13, which has a cysteinyl residue critical for the trypsin-like catalytic activity, as reported by L.R. Dick et al. [Biochemistry 31, 7347-7355, 1992], suggesting that RC10-II is a proteasomal subunit necessary for the expression of tryptic activity.

【 授权许可】

Unknown