Dramatic changes in control properties that accompany channelling and metabolite sequestration

FEBS Letters

Westerhoff, Hans V.²  Cascante, Marta³  Kholodenko, Boris N.¹ 

[1] Division of Molecular Biology, Netherlands Cancer Institute, H5, Plesmanlaan 121, 1066 CX Amsterdam, The Netherlands;E.C. Slater Institute, University of Amsterdam, Plantage Muidergracht 12, Amsterdam, The Netherlands;Department de Bioquimica i Fisiologia, Universitat de Barcelona, Marti i Franques 1, Barcelona, Spain

关键词: Metabolic channelling;    High enzyme concentrations;    Moiety conservation;    Metabolic control theory;   

DOI  :  10.1016/0014-5793(93)80841-H

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A simple summation theorem describes the control of fluxes in ‘ideal’ metabolic pathways. This paper shows how this theorem and the control properties of a pathway change when direct transfer of intermediates and/or sequestration of metabolites involved in moiety conservations (by enzymes present at high concentrations) take place. The derived generalized summation theorem quantifies the extent to which metabolite sequestration decreases and direct metabolite transfer can increase the control exerted by enzymes on the flux. The implications of metabolite channelling for the control of fluxes are discussed quantitatively.

【 授权许可】

Unknown