Crystallization and preliminary X‐ray analysis of leukemia inhibitory factor

FEBS Letters

Dauter, Zbigniew⁴  Betzel, Christian⁴  Hawkins, Nessa²  Weber, Wolfgang³  Pope, Joseph²  Fourme, Roger¹  Boone, Thomas²  Marnitz, Ulf³  Miller, James²  Samal, Babru²  Visanji, Marcia⁴ 

[1] LURE B209D, Universite' Paris Sud, 91405 Orsay Cedex, France;Amgen Inc., Amgen Center, Thousand Oaks, CA 91320, USA;Institut für Physiologische Chemie, Universitätskrankenhaus Eppendorf, 20251 Hamburg, Germany;EMBL, c/o DESY, Notkestraβe 85, 22603 Hamburg, Germany

关键词: Cytokine;    Isoelectric focusing;    Crystal;    Synchrotron radiation;   

DOI  :  10.1016/0014-5793(93)80810-H

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Leukemia inhibitory factor (LIF) is a polyfunctional molecule with significant and diverse biological activities. LIF is a glycoprotein secreted by a number of different cell types in vitro. It is induced in fibroblasts, lymphocytes, monocytes and astrocytes by various inducers such as serum, TNF, interleukin-IP and EGF. Due to extensive and variable glycosylation the molecular weight can range from 38 to 67 kDA. The biological functions of LIF are mediated through a receptor and a signal transducer, gp130, which is also used by factors like interleukm-6 (IL-6), cilliary neurotropic factor (CNTF), and oncostatin M (OSM). Here, we report the crystallization of the non-glycosylated human-like LIF expressed in E. coli. The present crystals diffract to 2.0 Å using synchrotron radiation. They belong to the monoclinic space group C2, and the cell dimensions are a = 61.5 Å, b = 45.3 Å , c =77.7 Å and β = 112.3°.

【 授权许可】

Unknown