Volume‐regulatory taurine release from a human lung cancer cell line

FEBS Letters

Kirk, Kiaran¹  Kirk, Julie¹ 

[1] University Laboratory of Physiology, Parks Road, Oxford, OX1 3PT, UK

关键词: Taurine;    Volume regulation;    RVD;    Volume-activated chloride channel;    Membrane transport;    DIDS;    4;    4'-diisothiocyanatostilbene-2;    2'-disulfonic acid;    HEPES;    N-(2-hydroxyethyl)piperazine-N-(2-ethanesulfonic acid);    MDCK;    Madin Darby Canine Kidney;    NPPB;    5-nitro-2-(3-phenylpropylamino) benzoic acid;    RVD;    regulatory volume decrease;   

DOI  :  10.1016/0014-5793(93)81630-I

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Exposure of a human lung epithelial cancer cell-line to hypo-osmotic media led to a marked increase in the rate of efflux from the cells of taurine, a non-essential sulfonic amino acid. The osmotically-activated taurine efflux was inhibited by a range of known Cl⁻ channel blockers, the most potent of which were NPPB and 1,9-dideoxyforskolin. These reagents were similarly effective at inhibiting the osmotically-activated efflux of I⁻, a known substrate of volume-activated Cl⁻ channels. The results are consistent with the hypothesis that volume-regulatory taurine release from these cells is mediated by a volume-activated Cl⁻ channel.

【 授权许可】

Unknown