Synthesis and farnesylation of a‐factor fusion proteins in Saccharomyces cerevisiae

FEBS Letters

Böttner, Benjamin¹  Hollenberg, Cornelis P.¹  Kölling, Ralf¹ 

[1] Institut für Mikrobiologie, Heinrich-Heine-Universität Düsseldorf, Universitätsstraße 1, D-40225 Düsseldorf, Germany

关键词: Secretion;    Mating;    Yeast;    Prenylation;    ABC transporters;   

DOI  :  10.1016/0014-5793(93)81625-A

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have generated in-frame fusions between the mouse dihydrofolate reductase (DHFR) and parts of the a-factor MFA1 gene to explore the potential of a-factor as a secretion signal for larger polypeptides. We demonstrated that the fusion proteins are farnesylated by comparing the mobility of fusion proteins prepared from a wild-type strain and a farnesyltransferase mutant (ste16/ram1) on SDS-gels and by an in vitro farnesylation assay. In contrast to unmodified DHFR, the fusion proteins could be sedimented from cell extracts by centrifugation. Solubilization experiments indicated that the highly hydrophobic a-factor moiety renders the fusion proteins insoluble, explaining why the fusions are not secreted into the culture medium.

【 授权许可】

Unknown