The presence of a calcium‐sensitive p26‐containing complex in bovine retina rod cells

FEBS Letters

Philippov, Pavel P.¹  Gorodovikova, Elena N.¹ 

[1] Department of Enzymology, A.N. Belozersky Institute of Physico-Chemical Biology, Moscow State University, Moscow 119899, Russian Federation

关键词: Photoreception;    Recoverin;    Guanylate cyclase;    Rhodopsin kinase;    Bovine retina rod cell;    ROS;    rod outer segments;    cGMP;    3';    5'-cyclic guanosine monophosphate;    Rh and Rh∗;    ‘dark’ and photobleached rhodopsin;    respectively;    GC;    guanylate cyclase;    PDE;    cGMP-phosphodiesterase;    RK;    rhodopsin kinase;   

DOI  :  10.1016/0014-5793(93)80746-H

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A protein with apparent M _r, 26 kDa (p26 [(1991) Biokhnya 56, 225-228] or recoverin [(1991) Science 251, 915-918]) was suggested to activate GC in a calcium dependent manner in bovine retina rod cells [(1991) Science 251, 915-918; (1991) EMBO J, 10, 793-798]. However, according to our present data homogeneous p26 preparations do not activate the enzyme. At the same time we have revealed a complex of p26 with an unidentified protein, presumably RK, in bovine ROS. Calcium favours formation of the complex whereas EGTA addition (which corresponds to a low free Ca²⁺ concentration) leads to its dissociation.

【 授权许可】

Unknown