The genomic region encoding toxin gamma from the scorpion Tityus serrulatus contains an intron

FEBS Letters

Martin, B.M.³  Bolivar, F.¹  Mejía, M.C.¹  Becerril, B.¹  Possani, L.D.¹  Lucas, S.²  Corona, M.¹ 

[1] Instituto de Biotecnología, Universidad Nacional Autónoma de México, Av. Universidad 2001, Apdo. Postal 510-3, Cuernavaca, 62271 México;Instituto Butantan, Av. Vital Brazil 1500, São Paulo, Brazil;National Institute of Mental Health, Molecular Neurogenetics Unit, Clinical Neuroscience Branch, Building 49, B1EE16, Bethesda, MD 20892, USA

关键词: Scorpion toxin;    Na+ channel;    Genomic DNA clone;    Nucleotide sequence;    Arachnid intron;    Tityus serrulatus;   

DOI  :  10.1016/0014-5793(93)80428-W

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The gene encoding toxin gamma from the scorpion, Tityus serrulatus, was amplified by PCR from genomic DNA employing synthetic oligonucleotides designed from the reported cDNA sequence. The nucleotide sequence of this gene reveals the presence of an intron of 475 base pairs (bp) which interrupts the region that encodes the signal peptide of the precursor toxin. A comparison of the intron boundary sequences of the gamma toxin gene with ones from other arachnid genes is also presented.

【 授权许可】

Unknown