Transcription in vitro promoted by the Agrobacterium VirG protein

FEBS Letters

Endoh, Hideki¹  Aoyama, Takashi¹  Oka, Atsuhiro¹ 

[1]Laboratory of Molecular Genetics, Institute for Chemical Research, Kyoto University, Uji-Shi, Kyoto-Fu 611, Japan

关键词: Agrobacterium;    Positive regulator;    Signal transduction;    Transcription;    in vitro;    Two-component regulatory system;    bp;    base-pair(s);    DTT;    dithiothreitol;    NTP;    ribonucleoside triphosphate (ATP;    GTP;    CTP and UTP);    pRi;    hairy-root-inducing plasmid;    pTi;    tumor-inducing plasmid;    vir;    virulence gene(s);    VirA;    protein encoded by vir A;    vir box;    6-bp sequence recognized by VirG;    VirG;    protein encoded by virG;   

DOI  :  10.1016/0014-5793(93)80694-P

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Expression of the virulence genes (vir) on the hairy-root-inducing plasmid pRiA4 is induced by plant signals in Agrobacterium cells through a two-component regulatory system, the VirA-VirG system. We constructed an in vitro transcription system that consisted of the purified VirG protein and the Agrobacterium RNA polymerase holoenzyme. Both versions of VirG, the non-phosphorylated form and the VirA-phosphorylated form, were active but showed different patterns of the pH-dependency for transcriptional activation.

【 授权许可】

Unknown