Studies on the ability of minor groove binders to induce supercoiling in DNA

FEBS Letters

Störl, K.²  Lown, J.W.¹  Zimmer, Ch.²  Burckhardt, G.² 

[1]Department of Chemistry, University of Alberta, Edmonton, Alba., Canada

[2]Department of Molecular Biology, Institute of Molecular Biology, Friedrich-Schiller-University, Winzerlaer Straße 10, 07708 Jena, Germany

关键词: Minor groove binder;    Plasmid DNA;    Supercoiling;    EB;    ethidium bromide;    CQ;    chloroquine;    Nt;    netropsin;    Nt-lmx;    netropsin analogues (lexitropsins) containing two (x = 2) or three (x = 3) N-methylimidazole residues;    bis-Nt-x (x = 5;    6;    8) bifunctional netropsins linked by x methylene residues;    Dst-3;    distamycin A;    Dst-2;    analogue of distamycin containing two N-methylpyrrole groups;    Dst-lm2;    distamycin containing two N-methylimidazole groups;    DAPI;    4′;    6-diamino-phenylindole;    SN-6999;    SN-16841;    SN-13282;    SN-6131;    SN-6132;    SN-18071;    bisquaternary ammonium heterocycles;   

DOI  :  10.1016/0014-5793(93)81678-S

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The effect of various non-intercalating minor groove binders on closed circular DNA in the presence of topoisomerase I has been studied by means of agarose gel electrophoresis. Analogues of the netropsin series (lexitropsins) and SN-6999 can effectively produce positive supercoils, as indicated by analysis of the topoisomers in the presence of chloroquine and the evaluated linking number changes. Analogues of the distamycin series are less effective, and bisquaternary ammonium heterocycles, as well as DAPI and pentamidine, were found to be ineffective ligands. The large differences observed in the ability of minor groove binders to induce positive supercoils are discussed.

【 授权许可】

Unknown