Calcium‐mobilizing effectors inhibit P‐enolpyruvate carboxykinase gene expression in cultured rat hepatocytes

FEBS Letters

Solanes, Gemma¹  Bosch, Fatima¹  Valera, Alfons¹ 

[1] Department of Biochemistry and Molecular Biology, School of Veterinary Medicine, Autonomous University of Barcelona, 08193-Bellaterra (Barcelona, Spain

关键词: Gene expression;    P-enolpyruvate carboxykinase;    Calcium ionophore A23187;    Phenylephrine;    Insulin;   

DOI  :  10.1016/0014-5793(93)80679-O

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Incubation of primary cultures of hepatocytes from fed and fasted rats with calcium ionophore strongly decreased glucose production from pyruvate. Like insulin, calcium ionophore A23187, phenylephrine, vasopressin, and prostaglandins E₂ and F_2α caused a significant reduction (50–60%) in basal concentrations of mRNA for P-enolpyruvate carboxykinase (PEPCK), the main regulatory enzyme of gluconeogenesis. Phenylephrine, prostaglandin E₂ and calcium ionophore A23187 were also able to counteract the induction of PEPCK gene expression by Bt₂cAMP. These effects were similar to those exerted by both vanadate and phorbol ester TPA. The decrease in extracellular calcium by the addition of the calcium-chelating agent EGTA to the incubation medium caused an increase in PEPCK mRNA levels. This effect was additive to that of Bt₂cAMP and was counteracted by vanadate.

【 授权许可】

Unknown