Heterocomplexes of tick‐borne encephalitis structural and non‐structural proteins

FEBS Letters

Pressman, E.K.¹ 

[1] Novosibirsk Institute of Bioorganic Chemistry of Siberian Division of Russian Academy of Sciences, Novosibirsk, Russian Federation

关键词: Tick-borne encephalitis virus;    Monoclonal antibody;    Immunoaffinity chromatography;    Complex structural and non-structural proteins;   

DOI  :  10.1016/0014-5793(93)80667-J

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The existence of the complex of structural and non-structural proteins in the tick-borne encephalitis (TBE) virus is shown. The complex was isolated from virus-containing cultural medium by immunoaffinity chromatography on monoclonal antibodies (MAbs). By enzyme immunoassay and immunoblotting with the use of appropriate MAbs it was demonstrated that this complex consists of structural (protein E), and non-structural (NS1) glycoproteins. Also, the trimer E-NS1-NS3 can be isolated. It is proposed that this trimer is the viral replicative complex.

【 授权许可】

Unknown