Cyclic nucleotide phosphodiesterase activity in Neurospora crassa Purification by immunoaffinity chromatography and characterization

FEBS Letters

Molina y Vedia, Luis¹  Rubinstein, Clara P.¹  Téllez-Iñón, María T.¹  Torres, Héctor N.¹  Ulloa, Rita M.¹ 

[1] Instituto de Investigaciones en Ingeniería Genética y Biología Molecular and Facultad de Ciencias Exactas y Naturales, Obligado 2490, 1428 Buenos Aires, Argentina

关键词: Monoclonal antibody;    Cyclic AMP phosphodiesterase;    Immunoaffinity chromatography;    (Neurospora crassa);   

DOI  :  10.1016/0014-5793(88)81065-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Monoclonal antibodies to Neurospora crassa cyclic nucleotide phosphodiesterase (PDE I) were selected by their capacity to inhibit the enzyme activity. The monoclonal immunoglobulin, coupled to Sepharose 4B, was used for the affinity purification of PDE I activity. After SDS-polyacrylamide gel electrophoresis the affinity purified PDE I fractions showed a single polypeptide band of about 41 kDa. This band reacted in Western blots with the above mentioned monoclonal immunoglobulin.

【 授权许可】

Unknown