Catabolite inactivation of the yeast maltose transporter is due to proteolysis

FEBS Letters

Lagunas, Rosario¹  Lucero, Pilar¹  Herweijer, Marga² 

[1] Instituto de Investigaciones Biomédicas de CSIC, Artuto Duperier 4, 28029 Madrid, Spain;Gist-Brocades, PO Box 1, 2600 MA Delft, The Netherlands

关键词: Catabolite inactivation;    Maltose transport;    Transport inactivation;    Transport turnover;    Saccharomyces cerevisiae;   

DOI  :  10.1016/0014-5793(93)80397-D

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The maltose transport capacity of fermenting Saccharomyces cerevisiae rapidly decreases when protein synthesis is impaired. Using polyclonal antibodies against a recombinant maltose transporter-protein we measured the cellular content of the transporter along this inactivation process. Loss of transport capacity was paralleled by a decrease of cross-reacting material which suggests degradation of the transporter. We also show that in ammonium-starved cells the half-life of the maltose transporter is 1.3 h during catabolism of glucose and > 15 h during catabolism of ethanol.

【 授权许可】

Unknown