Molecular structure of ras‐related small GTP‐binding protein genes of rice plants and GTPase activities of gene products in Escherichia coli

FEBS Letters

Umeda, Masaaki¹  Uchimiya, Hirofumi¹  Aotsuka, Satoshi¹  Kato, Atsushi²  Tsuge, Tomohiko¹  Anai, Toyoaki²  Kidou, Shin-ichiro¹ 

[1] Institute of Molecular and Cellular Biosciences, University of Tokyo, Bunkyo-ku, Tokyo 113, Japan;Department of Botany, Hokkaido University, Sapporo 060, Japan

关键词: cDNA;    Rice;    Small GTP-binding protein;    GTPase;    ypt/rab;   

DOI  :  10.1016/0014-5793(93)80650-J

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We isolated two rice cDNA clones (rid and ric2) encoding proteins homologous to the ras-related small GTP-binding protein. The amino acid sequences of rid and ric2 are conserved in four regions involved in GTP binding and hydrolysis which are characteristic in the ras and ras-related small GTP-binding protein genes. In addition, two consecutive cysteine residues near the carboxyl-terminal end required for membrane anchoring are also present in rid and ric2. The rid and ric2 proteins synthesized in Escherichia coli possessed GTPase activity (i.e. hydrolysis of GTP to GDP).

【 授权许可】

Unknown