Isolation and characterization of a rice cDNA encoding the γ‐subunit of translation elongation factor 1B (eEF1Bγ)

FEBS Letters

Tsukamoto, Shigenori¹  Ejiri, Shin-ichiro¹  Kidou, Shin-ichiro¹  Kobayashi, Satoru¹ 

[1] Cryobiosystem Research Center, Faculty of Agriculture, Iwate University, Ueda, Morioka, Iwate 020-8550, Japan

关键词: Elongation factor 1γ;    eEF1Bγ;    cDNA;    Translation;    Rice;   

DOI  :  10.1016/S0014-5793(98)01014-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We isolated a rice cDNA clone (refg) encoding the γ-subunit of translation elongation factor 1B (eEF-1Bγ; the old designation was EF-1γ). The refg encodes an open reading frame of 419 amino acids which shows a similarity to the equivalent sequences from animals and yeast. Complex formation analysis, which showed the recombinant protein of refg (His-eEF1Bγ) and formed a complex with GST-eEF-1Bβ, indicated that the refg encodes rice eEF1Bγ of the eEF1Bαβγ complex. Expression analysis showed that refg mRNA is very abundant in suspension-cultured cells during the exponential phase of growth. A DNA blot analysis indicated that refg is located at a single locus in the rice genome.

【 授权许可】

Unknown