FEBS Letters | |
In vitro activation of pro‐cathepsin B by three serine proteinases: leucocyte elastase, cathepsin G, and the urokinase‐type plasminogen activator | |
Guinec, Nathalie1  Dalet-Fumeron, Veronique1  Pagano, Maurice1  | |
[1] Laboratoire de biochimie, équipe associée à l'Ura 1460 CNRS, Faculté de Médecine Broussais Hotel-Dieu, Université Pierre et Marie Curie, 15 rue de l'école de médecine, 75270 Paris cedex 06, France | |
关键词: Pro-cathepsin B; Activation; Processing; Elastase; Cathepsin G; Urokinase; cathepsin B; EC 3.4.22.1; elastase; EC 3.4.21.11; cathepsin G; EC 3.4.21.20; urokinase; uPA; EC 3.4.21.31; Z; benzyloxycarbonyl; NH-Mec; 4 methyl-7-coumarylamide; EDTA; ethylene diamine tetraacetate; disodium salt; DTE; dithioerythritol; PBS; phosphate buffer saline; | |
DOI : 10.1016/0014-5793(93)80643-9 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
In vitro activation of pro-cathepsin B purified from ascitic fluid of ovarian carcinomas by serine proteinases was studied. Both elastase and cathepsin G from human leucocytes were found to be activators, on the basis of generation of cathepsin B activity and processing of the precursor. These results represent a new cooperative pathway between cancer cells and host cells. The urokinase-type plasminogen activator activated pro-cathepsin B faster than leucocyte proteinases. A new relationship is emerging between the cysteine proteinases and the plasmin-activation system. Both pathways suggest an important role of cathepsin B in the proteolytic cascade associated with tumour invasion.
【 授权许可】
Unknown
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