2‐Oxo‐histidine as a novel biological marker for oxidatively modified proteins

FEBS Letters

Uchida, Koji¹  Kawakishi, Shunro¹ 

[1] Laboratory of Food and Biodynamics, Nagoya University School of Agriculture, Nagoya 464-01, Japan

关键词: 2-Oxo-histidine;    HPLC-ECD;    Oxidative modification of protein;    Biological marker;    HPLC-ECD;    high performance liquid chromatography with electrochemical detection;   

DOI  :  10.1016/0014-5793(93)80632-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We report a promising finding that oxidative modification of proteins by free radicals could be monitored by the formation of oxidized histidine that is detectable by reverse-phase HPLC with electrochemical detection (HPLC-ECD). When the N-protected histidine derivative (N-benzoyl-histidine) was exposed to a free radical-generating system (copper/ascorbate), a number of products were detected by HPLC-ECD and the main product among them was found to be identical to N-benzoyl-2-oxo-histidine. The acid hydrolysis of N-benzoyl-2-oxo-histidine provided a single product (2-oxo-histidine) that was detected sensitively by HPLC-ECD. Thus 2-oxo-histidine was indeed generated as the main product in the oxidatively modified proteins by free radicals. Taken together, 2-oxo-histidine may be a useful biological marker for assessing protein modifications under oxidative stress.

【 授权许可】

Unknown