Characterization of the 97 and 103 kDa forms of starch branching enzyme from potato tubers

FEBS Letters

Ek, Bo¹  Rask, Lars¹  Larsson, Håkan¹  Khoshnoodi, Jamshid¹ 

[1] Uppsala Genetic Center, Department of Cell Research, Swedish University of Agricultural Sciences, Box 7055, S-75007 Uppsala, Sweden

关键词: Starch branching enzyme;    Q-enzyme;    Allozyme;    Potato tuber;    Solanum tuberosum;    Solanum commersonii;   

DOI  :  10.1016/0014-5793(93)80499-K

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

N-Terminal analysis, peptide mapping and partial peptide sequencing of the 97 and 103 kDa forms of starch branching enzyme from potato tubers showed that the two forms are highly related. A comparison with sequence data in the literature showed that these forms belong to the starch branching enzyme isoform I family. An internal cDNA fragment was obtained using PCR technology on potato tuber RNA with two oligonucleotide primers constructed from the peptide sequence data. Southern blot analysis using the PCR fragment as probe showed that there is only one gene locus encoding this isoform of the enzyme in Solanum tuberosum as well as in Solanum commersonii.

【 授权许可】

Unknown