An easy, simple inexpensive test for the specific detection of Pectobacterium carotovorum subsp. carotovorum based on sequence analysis of the pmrA gene

BMC Microbiology

Research Article

Mohamed Amdan¹  Moulay Mustapha Ennaji¹  Meriam Terta¹  Mohamed Kettani-Halabi²  El Mostafa El Fahime³  François Bouteau⁴ 

[1] Laboratoire de Virologie, Microbiologie et Qualité /Eco Toxicologie et Biodiversité, Université Hassan II Mohammedia – Casablanca, Faculté des Sciences et Techniques - Mohammedia - FSTM, BP 146, 20650, Mohammedia, Maroc;Laboratoire de Virologie, Microbiologie et Qualité /Eco Toxicologie et Biodiversité, Université Hassan II Mohammedia – Casablanca, Faculté des Sciences et Techniques - Mohammedia - FSTM, BP 146, 20650, Mohammedia, Maroc;Université Paris Diderot, Sorbonne Paris Cité, LEM Institut de Biologie des Plantes, 91405, Orsay, France;UATRS – CNRST, Angle Allal Fassi / FAR, 10 000, Hay Riad, Rabat, Maroc;Université Paris Diderot, Sorbonne Paris Cité, LEM Institut de Biologie des Plantes, 91405, Orsay, France;

关键词: pmrA;    Pectobacterium carotovorum;    Potato tuber;    Soft rot disease;    Genetic diversity;    Phylogenetic analysis;   

DOI  :  10.1186/1471-2180-13-176

 received in 2012-09-21, accepted in 2013-07-23,  发布年份 2013

来源: Springer

【 摘 要 】

BackgroundThe species Pectobacterium carotovorum includes a diverse subspecies of bacteria that cause disease on a wide variety of plants. In Morocco, approximately 95% of the P. carotovorum isolates from potato plants with tuber soft rot are P. carotovorum subsp. carotovorum. However, identification of this pathogen is not always related to visual disease symptoms. This is especially true when different pathogen cause similar diseases on potato, citing as an example, P. carotovorum, P. atrosepticum and P. wasabiae. Numerous conventional methods were used to characterize Pectobacterium spp., including biochemical assays, specific PCR-based tests, and construction of phylogenetic trees by using gene sequences. In this study, an alternative method is presented using a gene linked to pathogenicity, in order to allow accuracy at subspecies level. The pmrA gene (response regulator) has been used for identification and analysis of the relationships among twenty nine Pectobacterium carotovorum subsp. carotovorum and other Pectobacterium subspecies.ResultsPhylogenetic analyses of pmrA sequences compared to ERIC-PCR and 16S rDNA sequencing, demonstrated that there is considerable genetic diversity in P. carotovorum subsp. carotovorum strains, which can be divided into two distinct groups within the same clade.ConclusionspmrA sequence analysis is likely to be a reliable tool to identify the subspecies Pectobacterium carotovorum subsp. carotovorum and estimate their genetic diversity.

【 授权许可】

Unknown   
© Kettani-Halabi et al.; licensee BioMed Central Ltd. 2013. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.