Prevention of the induced synthesis and secretion of group II phospholipase A2 by brefeldin A

FEBS Letters

Sánchez, Rosa M.¹  Vervoordeldonk, Margriet J.B.M.¹  van den Bosch, Henk¹  Schalkwijk, Casper G.¹ 

[1] Centre for Biomembranes and Lipid Enzymology, Utrecht University, Padualaan 8, 3584 CH Utrecht, The Netherlands

关键词: 14 kDa group II phospholipase A2;    Brefeldin A;    Golgi structure;    Inhibition of protein synthesis and secretion;    Rat renal mesangial cell;   

DOI  :  10.1016/0014-5793(93)80492-D

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Brefeldin A (BFA) has previously been shown to block protein secretion and to cause dismantling of the Golgi cisternae in many cultured cell lines. BFA was found to prevent the induced synthesis and secretion of 14 kDa group II phospholipase A₂ (PLA₂) in rat mesangial cells. Furthermore, BFA inhibited total protein synthesis although PLA₂ appeared to be more sensitive to the effect of this compound than total protein synthesis assessed by amino acid incorporation. BFA was unable to block protein synthesis or PLA₂ activity in the cell completely but secretion of enzymatic activity and PLA₂ protein into the cell culture media was totally inhibited.

【 授权许可】

Unknown