FEBS Letters | |
Down‐regulation of monocytic VLA‐4 leads to a decreased adhesion to VCAM‐1 | |
Hakkarainen, Merja1  Majuri, Marja-Leena1  Mattila, Petri S.1  Renkonen, Risto1  Mattila, Pirkko1  Tiisala, Sinikka1  | |
[1] Department of Bacteriology and Immunology, PO Box 21 (Haartmaninkatu 3), 00014 University of Helsinki, Finland | |
关键词: Integrin; VLA-4; VCAM-1; Adhesion; VLA-4; very late activation antigen; VCAM-1; vascular cell adhesion molecule 1; ICAM-1; intercellular adhesion molecule-1; | |
DOI : 10.1016/0014-5793(93)80473-8 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
The α4β1 integrin VLA-4 is expressed on practically all leukocytes, except on mature granulocytes. Here we show that in vitro treatment of monocytic cells with phorbol-12-myristate-13-acetate (PMA) leads to a selective decrease in the VLA-4 α-chain expression, both at the RNA and protein level. Meanwhile the expression of β1 and that of α5 another α-chain associating with β1 was seen to increase. The decrease of α4 expression was restricted to monocytic cells, and was not observed on other VLA-4-positive cells tested (MOLT-4 T cells and HOS sarcoma cells). The down-regulation of the VLA-4 α-chain was followed by a decreased binding capacity of the cells to recombinant VCAM-1. This data indicates that while previous findings show that the integrin-dependent adhesion may rapidly be regulated by altering the avidity of the interacting molecules, their quantitative modulation also has a clear impact on adhesion.
【 授权许可】
Unknown
【 预 览 】
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