Down‐regulation of monocytic VLA‐4 leads to a decreased adhesion to VCAM‐1

FEBS Letters

Hakkarainen, Merja¹  Majuri, Marja-Leena¹  Mattila, Petri S.¹  Renkonen, Risto¹  Mattila, Pirkko¹  Tiisala, Sinikka¹ 

[1] Department of Bacteriology and Immunology, PO Box 21 (Haartmaninkatu 3), 00014 University of Helsinki, Finland

关键词: Integrin;    VLA-4;    VCAM-1;    Adhesion;    VLA-4;    very late activation antigen;    VCAM-1;    vascular cell adhesion molecule 1;    ICAM-1;    intercellular adhesion molecule-1;   

DOI  :  10.1016/0014-5793(93)80473-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The α₄β₁ integrin VLA-4 is expressed on practically all leukocytes, except on mature granulocytes. Here we show that in vitro treatment of monocytic cells with phorbol-12-myristate-13-acetate (PMA) leads to a selective decrease in the VLA-4 α-chain expression, both at the RNA and protein level. Meanwhile the expression of β₁ and that of α₅ another α-chain associating with β₁ was seen to increase. The decrease of α₄ expression was restricted to monocytic cells, and was not observed on other VLA-4-positive cells tested (MOLT-4 T cells and HOS sarcoma cells). The down-regulation of the VLA-4 α-chain was followed by a decreased binding capacity of the cells to recombinant VCAM-1. This data indicates that while previous findings show that the integrin-dependent adhesion may rapidly be regulated by altering the avidity of the interacting molecules, their quantitative modulation also has a clear impact on adhesion.

【 授权许可】

Unknown