Molecular cloning reveals the existence of a fourth γ subunit of the vertebrate brain GABAA receptor

FEBS Letters

Kim, Hye-Chin¹  Darlison, Mark G.¹  Harvey, Robert J.¹ 

[1] Institut für Zellbiochemie und klinische Neurobiologie, Universitäts-Krankenhaus Eppendorf, Universität Hamburg, Martinistrasse 52, 20246 Hamburg, Germany

关键词: cDNA cloning;    Chick brain;    GABA areceptor y subunit;    Developmental gene expression;    Hybridization;    in situ;    Visual processing;    Cbm;    cerebellum;    cDNA;    complementary DNA;    E;    ectostriatum;    GABA;    γ-aminobutyric acid;    HV;    hyperstriatum ventrale;    PCR;    polymerase chain reaction;    RSd;    nucleus reticularis superior;    pars dorsalis;    Rt;    nucleus rotundus;    TeO;    optic tectum;   

DOI  :  10.1016/0014-5793(93)80339-V

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have isolated a cDNA, from the chicken, that encodes a fourth type of γ subunit of the vertebrate brain GABA_A receptor. The mature polypeptide (which we name γ4) displays 67%, 69% and 70% identity, respectively, to the rat γ1, γ2 and γ3 subunits. In the developing chicken brain, the γ4-subunit mRNA is first detected at embryonic day 13; the transcript level then increases progressively during embryogenesis. In situ hybridization reveals that the γ4-subunit mRNA is abundant in several brain regions, including the ectostriatum, nucleus rotundus and hyperstriatum ventrale, which are involved in visual processing and learning.

【 授权许可】

Unknown