Identification of the protein and cDNA of the cardiac Na+ /H+ exchanger

FEBS Letters

Pouyssegur, Jacques²  Barr, Amy¹  Sardet, Claude²  Fliegel, Larry¹ 

[1] Cardiov. Dis. Res. Group, Department of Pediatrics and Biochemistry, University of Alberta, Edmonton, Alberta, T6G 2S2 Canada;Centre de Biochemie-CNRS, University of Nice, 06034 Nice, France

关键词: Na+ /H+ exchanger;    Sarcolemma;    cDNA cloning;   

DOI  :  10.1016/0014-5793(91)80241-T

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We examined the myocardial form of the Na⁺ /H⁺ exchanger. A partial length cDNA clone was isolated from a rabbit cardiac library and it encoded for a Na⁺ /H⁺ exchange protein. In comparison with the human Na⁺ /H⁺ exchanger, the sequence of the 5′ end of the cDNA was highly conserved, much more than the 3/t' region, while the deduced amino acid sequence was also highly conserved. To further characterize the myocardial Na⁺ /H⁺ exchange protein, we examined Western blots of isolated sarcolemma with antibody produced against a fusion protein of the Na⁺ /H⁺ exchanger. The antibodies reacted with a sarcolemma protein of 50 kDa and with a protein of 70 kDa. The results show that the rabbit myocardium does possess a Na⁺ /H⁺ exchanger protein homologous to the known human Na⁺ /H⁺ exchanger.

【 授权许可】

Unknown