ras oncogene‐induced transformation of a rat seminal vesicle epithelial cell line produces a marked increase of adenylate cyclase and protein kinase C activities

FEBS Letters

Chiosi, E.¹  Illiano, G.¹  Metafora, S.²  Furgi, A.²  Valente, F.¹  Marchese, M.²  Naviglio, S.¹  Spina, A.M.¹ 

[1] Department of Biochemistry and Biophysics, 2nd University of Naples, via Costantinopoli 16, 80138 Naples, Italy;CNR International Institute of Genetics and Biophysics, via G. Marconi 10, 80125 Naples, Italy

关键词: ras oncogene;    Cell transformation;    G-protein;    Adenylate cyclase;    Protein kinase C;   

DOI  :  10.1016/0014-5793(93)80315-L

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Cells transformed by Kirsten murine sarcoma virus (Ki-MSV) have basal adenylate cyclase activity (AC) higher than control cells and comparable level of forskolin-stimulated AC activity. Moreover, a higher protein kinase C (PKC) activity was found to be present in the transformed cells. The molecular mechanism underlying the increase of AC activity was investigated. Our findings strongly suggest that this biochemical event is due to a marked decrease of the α_i negative control of the enzyme, even though the α_i of transformed cells appears to possess fully functional domains interacting with both the effector enzyme and the agonist-activated receptor.

【 授权许可】

Unknown