Adenosine stimulates 5'‐nucleotidase activity in rat mesangial cells via A2 receptors

FEBS Letters

Vlahovic, P.¹  Stefanovic, V.¹  Ardaillou, R.¹  Savic, V.¹  Ardaillou, N.¹ 

[1] INSERM 64, Hôpital Tenon, 4 rue de la Chine, 75020 Paris, France

关键词: 5'-Nucleotidase;    Rat;    Mesangial cell: Adenosine;    Cyclic AMP;   

DOI  :  10.1016/0014-5793(93)80304-D

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Because A₂ adenosine receptor activation stimulates adenylate cyclase and cyclic AMP induces 5'-nucleotidase expression in rat mesangial cells, we examined the effect of adenosine and its analogs on 5'-nucleotidase activity in these cells. A₂ adenosine receptors were characterized using [³H]5'-N-ethylcarboxamidoadenosine (NECA) as a tracer. There was a single group of receptor sites with a K _D value of 0.53 μM and a number of sites of 1,317 fmol/mg. [³H]NECA binding was inhibited preferentially by A₂ adenosine analogs and antagonists. Similarly, the order of potency for cAMP stimulation was in favour of A₂ adenosine analogs. Rat mesangial cells expressed surface 5'-nucleotidase activity. Exposure of cells for 48 h to adenosine analogs showed that at low concentrations A₂ analogs stimulated 5'-nucleotidase activity. These results indicate that adenosine upregulates activity of 5'-nucleotidase, the enzyme responsible for its local formation, via A₂ receptor stimulation and increase in cAMP production.

【 授权许可】

Unknown