Hormonal Regulations of Alkaline Phosphatase, 5'-Nucleotidase and γ-Glutamyltransferase Activities in Adult Rat Hepatocytes Cultured in Serum-Free Medium Collagen Gel

Cell Structure and Function

Yoshifumi Horiuti¹  Kazuo Yano¹  Yuzo Shibuya¹ 

[1] Asahi Chemical Industry Co., Ltd. Institute for Life Science Research

关键词: Hepatocyte;    Hormonal regulation;    Alkaline phosphatase;    5'-Nucleotidase;    γ-Glutamyltransferase;    Growth hormone;   

DOI  :  10.1247/csf.18.73

学科分类：分子生物学，细胞生物学和基因

来源: Japan Society for Cell Biology

【 摘 要 】

References(31)Cited-By(1)Primary hepatocytes were cultured on collagen gel in serum-free, α-modified Eagle's minimum essential medium containing 0.1 μMinsulin, 0.1 μM dexamethasone, 10 mM pyruvate and supplements such as glucagon, epinephrine or growth hormone. The activities of alkaline phosphatase, 5' -nucleotidase and γ-glutamyltransferase were assayed in cell extracts prepared from the cultures. All three enzyme activities were induced by glucagon, epinephrine or dibutyryl cAMP.The maximally effective concentration of glucagon was 5-10 nM for both alkaline phosphatase and 5' -nucleotidase and 100 nMfor γ-glutamyltransferase. Only alkaline phosphatase activity was suppressed by growth hormone, which caused marked suppression at about 1 μU (0.25 ng)/ml. Taurocholate also induced both alkaline phosphatase and γ-glutamyltransferase activities at 1 mM.

【 授权许可】

Unknown