The effect of cyclic AMP analogues and glucagon on cholesteryl ester synthesis and hydrolysis in cultured hamster hepatocytes

FEBS Letters

Hoang, Van-Quyen¹  Suckling, Keith E.²  M. Botham, Kathleen¹  Cho-Chui, Y.S.² 

[1] Department of Veterinary Basic Sciences, The Royal Veterinary College, Royal College St., London, NW1 OTU, UK;SmithKline Beecham Pharmaceuticals, The Fry the, Welwyn, Herts., UK and c National Cancer Institute, Laboratory of Tumour Immunology and Diagnosis, Bethesda, MA, USA

关键词: Cholesterol esterification;    Cholesteryl ester hydrolysis;    Cyclic AMP analogue;    Glucagon;    Hamster hepatocyte;    ACAT;    acyl Co-enzyme A:cholesterol acyl transferase;    cAMP;    cyclic AMP;    8-C1 cAMP;    8-chloro cyclic AMP;    8-CPT cAMP;    8-(4 chlorophenylthio) cyclic AMP;   

DOI  :  10.1016/0014-5793(93)80183-U

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Two cyclic AMP analogues, 8-chloro cyclic AMP and 8-(4 ehlorophenylthio) cyclic AMP, were found to increase the incorporation of [³H]oleate into cholereryl ester in cultured hamster hapatocytes (30–40%), while incorporation into triacylglycerol was unaffected. An increase of a similar magnitude was observed in the presence of glucagon and the phosphodiesterase inhibitor, theophylline. The cyclic AMP analogues also stimulated the activity of neutral cholesteryl ester hydrolase in the cells, and this effect was mimicked by glucagon and theophylline. These results show that cyclic AMP can affect the cholesteryl ester cycle in hamster hepatocytes, and support the idea that the enzymes involved may be co-ordinately regulated.

【 授权许可】

Unknown