Osteoblast‐like cells have a variable mixed population of purino/nucleotide receptors

FEBS Letters

Ferrier, Jack¹  Yu, Hesheng¹ 

[1] Medical Research Council Group in Periodontal Physiology, University of Toronto, Toronto, Ontario, M5S 1A8, Canada

关键词: Osteoblast;    ATP;    Purinergic receptor;    Nucleotide receptor;    Intracellular calcium;    Cross-inhibition;   

DOI  :  10.1016/0014-5793(93)80994-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Osteoblast-like UMR 106.06 cells respond to extracellular application of nucleotides with a fast intracellular calcium pulse (latency of about 20 s, half-width of about 10 s), as measured with fluo-3 on a confocal laser scanning system. Cross-inhibition experiments at 50 μM show that, on a cell population basis, adenosine triphosphate (ATP) strongly inhibits the effect of uridine triphosphate (UTP) or 2-methylthio-ATP (2-MeSATP) applied within 2 min after the end of the ATP-induced pulse, while prior application of UTP or 2-MeSATP only weakly inhibits the ATP effect, and UTP and 2-MeSATP weakly inhibit each other. Furthermore, there are clear differences in cross-inhibition responses between individual cells. Our measurements provide strong evidence that these cells have at least two types of purino/nucleotide receptors, probably P_2y and P_2u, with a proportion that varies between individual cells.

【 授权许可】

Unknown