Translation and fates of the gag protein of 1731, a Drosophila melanogaster retrotransposon

FEBS Letters

Coulondre, Christine²  Best-Belpomme, Martin²  Kim, Myeong-Hee²  Champion, Serge¹  Lacoste, Jérôme²  Maisonhaute, Claude² 

[1] Institut de Chimie Biologique, Université Aix Marseille I, case 31, 13331 Marseille Cedex 03, France;Groupe de Génétique Cellulaire et Moléculaire, URA-CNRS 1135, 7 Quai Saint Bernard, 75005 Paris, France

关键词: 1731 retrotransposon;    Drosophila;    Gag protein;    Antisense;   

DOI  :  10.1016/0014-5793(93)80989-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

An entire copy of 1731, a Drosophila melanogaster retrotransposon, was tagged by fusing in frame its putative gag gene with the reporter LacZ sequence. The high transfection efficiency of Drosophila virilis cells added to the absence of 1731 in their genome allowed, by combining histochemical staining and immunological detections, the demonstration of the translation of the 1731 gag gene. The gag protein is gathered in virus-like particles. Its occurrence in nuclei is consistent with a nuclear localization signal. The expression of the sense construction was inhibited by cotransfections with its antisense homologue.

【 授权许可】

Unknown