The regulated expression of an intrabody produces a mutant phenotype in Drosophila

FEBS Letters

De Baetselier, Patrick¹  Devoogdt, Nick¹  Hassanzadeh Gh, Gholamreza¹  Muyldermans, Serge¹  Dambly-Chaudière, Christine²  Ghysen, Alain² 

[1]Department of Ultrastructure, Immunology and Parasitology, Vlaams Interuniversitair Instituut voor Biotechnologie, Vrije Universiteit Brussel, Paardenstraat 65, B-1640 Sint-Genesius-Rode, Belgium

[2]Laboratoire de Neurogénétique, CC103, Université de Montpellier II, Place E. Bataillon, 34095 Montpellier, France

关键词: Poxn;    Phenocopy;    Intrabody;    Antisense;    Drosophila;    CNS;    central nervous system;    es organ;    external sense organ;    m-es organ;    mono-innervated external sense organ;    PCR;    polymerase chain reaction;    p-es organ;    poly-innervated external sense organ;    PNS;    peripheral nervous system;    scFv;    single-chain variable fragment;   

DOI  :  10.1016/S0014-5793(98)01205-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Intrabodies show great promise for controlling gene expression. As an initial attempt to evaluate the intrabody technology in Drosophila, the gene poxn was used as target. Transgenic flies harboring different anti-Poxn scFv genes integrated into various chromosomes were obtained. In one transformant, a phenocopy resembling the hypomorphic poxn ⁻ phenotype was produced in embryos and larvae following induction of expression of α-Poxn2 intrabody. The antisense approach was used as control. Parameters that can affect the success of intrabody technology are described.

【 授权许可】

Unknown