Transcription and translation of inducible nitric oxide synthase in the pancreas of prediabetic BB rats

FEBS Letters

Kolb-Bachofen, Victoria³  Nathan, Carl²  Martin, Stephan¹  Xie, Qiao-wen²  Kolb, Hubert¹  Kleemann, Rainer¹  Rothe, Helga¹ 

[1] Diabetes Research Institute, University of Düsseldorf, D-40225 Düsseldorf, Germany;Department of Medicine, Cornell University Medical College, New York, NY, USA;Institute for Immunobiology, University of Düsseldorf, D-40225 Düsseldorf, Germany

关键词: Diabetes type 1;    BB rat;    Insulitis;    NO synthase;    Nitric oxide;    iNOS;    inducible NO synthase;    cNOS;    constitutive NOS;   

DOI  :  10.1016/0014-5793(93)80954-S

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The inducible NO synthase (iNOS) was found to be expressed in pancreatic lesions of adult diabetes-prone BB rats. Pancreatic iNOS mRNA was detected by reverse transcriptase PCR in pancreatic RNA of adult diabetes-prone BB rats but not in normal Wistar rats, young diabetes-prone BB rats without insulitis or in diabetes-resistant BB rats. Immunohistochemistry of pancreatic sections using an iNOS-specific antiserum labeled the pancreas of adult diabetes-prone BB rats but not Wistar rats. Parallel staining for EDI-positive macrophages showed restriction of iNOS expression to areas of islet infiltration by macrophages. In conclusion, the data provide direct evidence for enhanced expression of inducible NO synthase in tissue lesions during the development of autoimmune diabetes.

【 授权许可】

Unknown