Drastic reduction of the zinc‐ and magnesium‐stimulated protein tyrosine kinase activities in Alzheimer's disease hippocampus

FEBS Letters

Burbaeva, Gulnur Sh.²  Vener, Alexander V.¹  Aksenova, Marina V.² 

[1] A.N. Belozersky Institute of Phvsico-Chemical Biology, Moscow State University, Moscow, Russian Federation;National Research Center for Mental Health, Russian Academv of Medical Sciences, Moscow, Russian Federation

关键词: Protein phosphorylation;    Phosphotyrosine;    Zinc;    Magnesium;    Human hippocampus;    Alzheimer's disease;   

DOI  :  10.1016/0014-5793(93)80953-R

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Tyrosine phosphorylation of proteins from postmortem hippocampi of five Alzheimer's disease and five control cases have been compared. It was found that addition of Zn²⁺ or Mg²⁺ to membrane fractions of control hippocampi caused the phosphorylation of 32-, 40-, 55-, 60-, 80- and 100-kDa proteins or 43-, 55-, 60- and 90-KdA proteinsrespectively. The phosphorylation of all these proteins is shown to be drastically reduced in Alzheimer's disease hippocampi. Vanadate an inhibitor of protein tyrosine phosphataseshad no influence on the level of protein phosphorylation. Western blot analysis did not reveal any differences in the anti-phosphotyrosine immunoreactive membrane proteins from Alzheimer's disease and control hippocampi. Tyrosine kinase activity of immunoprecipitated p60^c-src from Alzheimer's disease and control hippocampi were the same. In conclusion, the Zn²⁺- and Mg²⁺ -stimulated tyrosine kinase activities distinct from activity of p60^c-src are decreased in Alzheimer's disease hippocampus.

【 授权许可】

Unknown