Antisem to carcinoembryonic antigen recognizes a phosphotyrosine‐containing protein in human colon cancer cell lines

FEBS Letters

Skubitz, Keith M.¹  Skubitz, Amy P.M.²  Goueli, Said A.²  Ducker, Thomas P.¹ 

[1] Department of Medicine, The University of Minnesota Medical School, and the Masonic Cancer Center, Minneapolis, MN 55455, USA;Department of Laboratory Medicine and Pathology, The University of Minnesota Medical School, and the Masonic Cancer Center, Minneapolis, MN 55455, USA

关键词: Biliary glycoprotein;    Carcinoembryonic antigen;    Ectoprotein kinase;    Protein phosphorylation;    Phosphotyrosine;    CEA;    carcinoembryonic antigen;    NCA;    non-specific cross reacting antigen;    BGP;    biliary glycoprotein;    FBS;    fetal bovine serum;    PMSF;    phenylmethylsulfonylfluoride;    NRS;    normal rabbit serum;    PAGE;    polyacrylamide gel electrophoresis;    P-S;    phosphoserine;    P-T;    phosphothreonine;    P-Y;    phosphotyrosine;   

DOI  :  10.1016/0014-5793(93)80021-L

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Members of the carcinoembryonic antigen (CEA) family include CEA, non-specific cross reacting antigen (NCA), and biliary glycoprotein (BGP), and appear to function as cell adhesion molecules. Immunoprecipitation and subsequent gel electrophoresis of proteins from several colon cancer cell lines labeled with [γ-³²P]ATP, under conditions designed to detect ecto-kinase-catalyzed phosphorylation of cellular proteins, revealed that polyclonal anti-CEA antiserum recognized a 175–190 kDa phosphoprotein on the surface of colon cancer cells. The ability to detect this phosphoprotein did not correlate with CEA production, and immunoprecipitation studies suggested that the phosphoprotein is BGP. Phosphoamino acid analysis of the 175–190 kDa protein showed that it contained predominantly phosphotyrosine.

【 授权许可】

Unknown