Possible regulation of CFTR‐chloride channels by membrane‐bound phosphatases in pancreatic duct cells

FEBS Letters

Figarella, C.²  Hollande, E.³  Becq, F.¹  Merten, M.²  Fanjul, M.³  Gola, M.¹ 

[1] Laboratoire de Neurobiologie, CNRS, LNB4, 31 chemin Joseph Aiguier, 13402 Marseille, France;Groupe de Recherche sur les Glandes Exocrines, 27 boulevard Leï Roure, 13009 Marseille, France;Laboratoire de Biologie Cellulaire, Université Paul Sabatier, 38 rue des Trente-Six-Ponts, 31400 Toulouse, France

关键词: CFTR-chloride channel;    Phosphatase;    Pancreas;    CF;    cystic fibrosis;    CFTR;    cystic fibrosis transmembrane conductance regulator;    PCR;    polymerase chain reaction;    AP;    alkaline phosphatase;    PKA;    protein kinase A;   

DOI  :  10.1016/0014-5793(93)81016-S

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have studied CFTR-Cl⁻ channels in non-CF CAPAN-1 and in CFTR-transfected CFPAC-PLJ-CFTR-6 epithelial cells from human pancreas. Theophylline and IBMX induced the opening of cell-attached CFTR-Cl⁻ channels. Theophylline, IBMX and the alkaline phosphatase (AP) inhibitor levamisole enhanced the activity of excised channels and reduced by 70–75% the apical membrane-associated APs activity. Okadaic acid had no effect on APs and channel activities. A polyclonal anti-alkaline phosphatase antibody (which detected apical APs) reduced APs activity and activated quiescent excised chloride channels. These results suggest that CFTR channels may be regulated by membrane-bound phosphatases.

【 授权许可】

Unknown