Probing the active center of the mitochondrial dicarboxylate transporter

FEBS Letters

Sholtz, K.F.¹  Mamaev, D.V.¹  Bondarenko, D.I.¹ 

[1] A.N. Bach Institute of Biochemistry, Russian Academy of Sciences, Moscow 117071, Russian Federation

关键词: Dicarboxylate transporter;    2-n-Alkylmalonates;    Active center;   

DOI  :  10.1016/0014-5793(93)81038-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

2-n-Alkylmalonates with various length of the alkyl residue have been used to study the topography of the active center of the dicarboxylate transporter in intact rat liver mitochondria. Measurements of the K_i values of these competitive inhibitors suggest that in the transporter there is a large hydrophobic region at least 1.7 nm in size, containing a polar domain (ca. 0.5 nm) and situated close to a substrate-binding site. These zones are assumed to be involved in the mechanism of dicarboxylate transport.

【 授权许可】

Unknown