Inhibition of the replication of native and 3′‐azido‐2′,3′‐dideoxy‐thymidine (AZT)‐resistant simian immunodeficiency virus (SIV) by 3‐nitrosobenzamide

FEBS Letters

Kun, Ernest²  Schaeffer, Catherine A.¹  Chuang, Ronald Y.³  Mendeleyev, Jerome²  Rice, William G.¹  Chuang, Ann J.³  Killam, Keith F.³ 

[1] Laboratory of Antiviral Drug Mechanisms, Program Resources Inc./Dyn-corp., National Cancer Institute-Frederick Cancer Research and Development Center, Building 431, PO Box B, Frederick, MD 21702, USA;Octamer Research Foundation and the Laboratory for Environmental Toxicology and Chemistry, Romberg Tiburon Centers, San Francisco State University, PO Box 855, Tiburon, CA 94920, USA;Department of Medical Pharmacology and Toxicology, School of Medicine, University of California, Davis, CA 95616, USA

关键词: AZT-resistant SIV;    6-Nitrosobenzamide;    Antiviral;    Zinc finger;    Anti-AIDS;    AZT;    3′-azido-2′;    3′-dideoxythymidine;    HIV;    human immunodeficiency virus;    MMU;    Macaca mulatta;    MTT;    3-(4;    5-di-methylthiazol-2-yl)-2;    5-diphenyltetrazolium bromide;    NOBA;    3-nitrosobenzamide;    NOBP;    6-nitroso-1;    2-benzopyrone;    PBMCs;    peripheral blood mononuclear cells;    PCR;    polymerase chain reaction;    PHA-PBL;    phytohaemagglutinin-stimulated human peripheral blood lymphocytes;    RT;    reverse transcriptase;    SIV;    simian immunodeficiency virus;    SIVsmm and SIVmmbi are SIV strains;    TCID50;    50% tissue culture infectious dosage;   

DOI  :  10.1016/0014-5793(93)81778-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The 3-nitrosobenzamide (NOBA) drug abolishes SIV replication sharply at 20 μM concentration when CEM × 174 cells are preincubated for 1 h with the drug prior to viral infection. Treatment of CEM × 174 cells with 20 μM NOBA resulted in the inhibition of the synthesis of the DNA sequence coding for the gag gene, as determined by the PCR technique. Cell viability was directly proportional to the antiviral action of NOBA. Replication of AZT-resistant SIV 23740 in MMU 23740 cells in vitro was suppressed by NOBA in a concentration-dependent manner without significant effects on cell viability. Reverse transcriptase activity of SIV_mac239 was unaffected by NOBA up to 800 μM concentration. Preincubation of two SIV strains with NOBA completely abolished their infectivity in human PHA-PBL cells. Replication of two strains of SIV in PHA-PBL cells was also inhibited by NOBA.

【 授权许可】

Unknown