Indoleacetic acid pretreatment of tobacco plants in vivo increases the in vitro sensitivity to auxin of the plasma membrane H+‐ATPase from leaves and modifies the polypeptide composition of the membrane

FEBS Letters

Vansuyt, Gérard¹  Santoni, Véronique¹  Rossignol, Michel¹ 

[1] Lab. Biochimie et Physiologie Végétales, INRA/ENSA-M/CNRS UA 573, 34060 Montpellier Cedex 1, France

关键词: Auxin;    Plasma membrane H+-ATPase;    Proton translocation;    Auxin-responsive polypeptides;   

DOI  :  10.1016/0014-5793(93)81751-K

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The sensitivity to auxin of the H⁺-ATPase-mediated proton translocation was investigated in vitro using purified plasma membrane vesicles from tobacco leaves. In vivo pretreatment of tobacco plants with auxin promotes a 100-fold increase of the in vitro sensitivity to auxin. This effect is specific for biologically active auxins and is dose- and time-dependent. In addition, pretreatment with auxin induces the accumulation of several polypeptides in the plasma membrane. These polypeptides constitute the first set of hormone-responsive polypeptides evidenced in plant membranes.

【 授权许可】

Unknown