rol A modulates the sensitivity to auxin of the proton translocation catalyzed by the plasma membrane H+‐ATPase in transformed tobacco

FEBS Letters

Tepfer, Mark²  Vansuyt, Gérard¹  Vilaine, Françoise²  Rossignol, Michel¹ 

[1] INRA, Physiopathologie, Végétale, B. V. 1540, F21034 Dijon Cedex, France;INRA, Biologie Cellulaire, F78026 Versailles Cedex, France

关键词: rol A gene;    Auxin;    Plasma membrane H+-ATPase;    Flowering;   

DOI  :  10.1016/0014-5793(92)80028-F

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In order to investigate the effect of the rol A gene product on the plasma membrane response to auxin, a clone of rol A-transformed tobacco was prepared. Auxin sensitivity was measured by the auxin concentration which induced the highest stimulation of H⁺-ATPase-mediated proton translocation on isolated plasma membrane vesicles. Both transformed and control plants exhibited identical auxin-sensitivity changes during vegetative and induction to flowering periods. However the sensitivity of flowering-transformed plants Was 100-times higher than that of control plants. Consistent observations were also made when using rol A+B+C-transformed plants. These results suggest that the rol A gene product could either participate in or affect the reception/transduction pathway of auxin signal at the plasma membrane.

【 授权许可】

Unknown