Pre‐translational regulation of lipid synthesizing enzymes and surfactant proteins in fetal rat lung in explant culture

FEBS Letters

Fraslon, C.¹  Batenburg, J.J.¹ 

[1] Laboratory of Veterinary Biochemistry, Utrecht University, Utrecht, The Netherlands

关键词: Lung surfactant;    Glucocorticoid;    Lipogenic enzyme;    Fatty acid synthase;    CTP:phosphocholine cytidylyltransferase;    Surfactant protein;   

DOI  :  10.1016/0014-5793(93)81090-M

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In hormone-free explant cultures of 18-day fetal rat lung the levels of the mRNAs for fatty acid synthase, ATP citrate lyase and surfactant proteins A, B, and C, increased as they do in vivo. Also CTP:phosphocholine cytidylyltransferase mRNA increased spontaneously. Unlike in vivo, malic enzyme mRNA increased drastically in cultured explants. Culture with dexamethasone increased the abundance of fatty acid synthase and surfactant protein mRNAs, but considerably depressed that of malic enzyme mRNA. Dexamethasone had little effect on CTP:phosphocholine cytidylyltransferase mRNA, supporting the concept that the increased activity of this enzyme caused by glucocorticoid is due to increased fatty acid synthesis.

【 授权许可】

Unknown